pkm2 expression plasmid (Sangon Biotech)
90
Structured Review
Sangon Biotech
pkm2 expression plasmid
Pkm2 Expression Plasmid, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkm2+expression+plasmid/sirnas/pmc11352541-79-1-7
Average 90 stars, based on 1 article reviews
Pkm2 Expression Plasmid, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkm2+expression+plasmid/sirnas/pmc11352541-79-1-7
Average 90 stars, based on 1 article reviews
pkm2 expression plasmid - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway"
Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway
Journal: International Journal of Chronic Obstructive Pulmonary Disease
doi: 10.2147/COPD.S468825
Figure Legend Snippet: The Sequences of the Pkm2-PACH
Techniques Used: Sequencing
Figure Legend Snippet: Rat Primer Sequences for qRT-PCR
Techniques Used: Sequencing
Figure Legend Snippet: Human Primer Sequences for qRT-PCR
Techniques Used: Sequencing
Figure Legend Snippet: The effects of CSE on mitochondrial oxidative damage and Pkm2/Nrf2 signaling pathway in A549 cells. ( A and C ) The effects of 10%CSE induction for 6 hours, 12 hours, and 24 hours on the generation of mROS in A549 cells. ( B ) Inhibition of mitochondrial membrane potential after 10%CSE induction for 6 hours, 12 hours, and 24 hours. ( D and E ) Inhibition of mitochondrial respiratory chain complex I and III after 10%CSE induction for 6 hours, 12 hours, and 24 hours. ( F and G ) Inhibition of T-SOD and GSH-Px after 10%CSE induction for 6 hours, 12 hours, and 24 hours. ( H ) Western blot analysis for assessing the effects of mitochondrial-related pathway including caspase-3, Bcl-2, Bax, and Cyt-C in A549 cells following 10%CSE induction. ( I ) Quantitative real-time PCR analysis for assessing the effects of Pkm2/Nrf2 signaling pathway including Pkm2, Nrf2, GCLC, and GCLM in A549 cells following 10%CSE induction. ( J ) Western blot analysis for assessing the effects of Pkm2/Nrf2 signaling pathway including Pkm2, Nrf2, GCLC, and GCLM in A549 cells following 10%CSE induction. All data are represented as mean ± SD (n=3). * P < 0.05 and ** P < 0.01 as compared to Con group, # P < 0.05 and ## P < 0.01 as compared to 10%CSE-6h group, and ■ P < 0.05 and ■■ P < 0.01 as compared to 10%CSE-12h group.
Techniques Used: Inhibition, Membrane, Western Blot, Real-time Polymerase Chain Reaction
Figure Legend Snippet: Mitochondria can be protected from oxidative damage by activating the Pkm2/Nrf2 signaling pathway. ( A (i) and (ii)) The production of mtROS after treated for Pkm2 expression plasmid in A549 cells. ( B – D ) Changes in mitochondrial membrane potential, and enzymatic activities of mitochondrial respiratory chain complexes I and III. ( E and F ) The activity of T-SOD and GSH-Px with Pkm2 expression plasmid. ( G ) Western blot analysis for assessing the effects of Pkm2/Nrf2 signaling pathway including Pkm2, Nrf2, GCLC, and GCLM in A549 cells following Pkm2 expression plasmid transfection. All data are represented as mean ± SD (n=3). * and ** indicate P < 0.05 and P < 0.01.
Techniques Used: Expressing, Plasmid Preparation, Membrane, Activity Assay, Western Blot, Transfection
Figure Legend Snippet: Effects of ECC-BYF III on Pkm2/Nrf2 signaling pathway in lung tissue of COPD rats. ( A – D ) Pkm2, Nrf2, GCLC and GCLM mRNA expression in lung tissue; ( E – I ) Pkm2, Nrf2, GCLC and GCLM levels as assessed by Western blot. All data are represented as mean ± SD (n=3). * P < 0.05 and ** P < 0.01 as compared to Normal group, # P < 0.05 and ## P < 0.01 as compared to Model group.
Techniques Used: Expressing, Western Blot
Figure Legend Snippet: Schematic diagram of ECC-BYF III regulates Pkm2/Nrf2 signaling pathway.
Techniques Used:
Related Articles
Expressing:Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway Article Snippet: .. The Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway Article Snippet: .. Effects of Plasmid Preparation:Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway Article Snippet: .. The Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway Article Snippet: .. Effects of Synthesized:Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway Article Snippet: .. The Article Title: Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway Article Snippet: .. Effects of |